Composite

Part:BBa_K1891002:Design

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-10)


Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 435
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 589
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence of luciferase gene is from NCBI (Genebank:M65067.1). A pBAD promoter(BBa_K206000) and a RBS(BBa_B0034) are added upstream the functional gene to produce this enzyme. Lociferase-pSB1C3 plasmid is transformed into E. coli strain transB (DE3) to express the protein. It can show fluorescence when we add its substrate fluorescein.


Source

We get this gene sequence from NCBI (Genebank:M65067.1), and then we get the DNA fragment from a synthesis company.

References